Sentence examples for solution was chilled from inspiring English sources
The solution was chilled at −80°C for 5 min and heated at 65°C for 5 min, which was repeated for three times.
The solution was chilled on ice overnight at 4 °C prior to centrifugation at 11,000 g for 15 minutes.
The solution was chilled and 15 U RNAse I (from the Roche cDNA synthesis kit) was added.
The solution was chilled on ice before TFMSA/TFA (1 4, v/ v; 1.63 mL) was added.
The solution was chilled on ice and diluted 1 100 with 10 mM Tris, pH 7.5, 0.05 mM EDTA and used directly in qPCR.
The cell/Matrigel solution was chilled on ice before injection, and each animal was injected with an equal number of OSCC cells (2.5 × 10) submucosally in the floor of the mouth.
Fixative solutions were chilled, then injected for 10 min with a peristaltic pump at a flow rate of 10 ml/min.
The solutions were chilled to 4° and centrifuged for 15 min at 40,000 rpm in a Spinco fixed-angle 40 rotor at 4°.
The buffer solution with chymotrypsin was chilled at 4 °C, and 10 μL of peptide solution was added to 1 mL of a chilled buffer solution to initiate the reaction.
Ethanol (900 μL) was added to the digestion solution, and the sample was chilled at 0 °C for 10 min. The sample solution was centrifuged at 14000 rpm for 10 min at 4 °C.
