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Exact(55)
Excess PLO solution was aspirated; then, the surface was rinsed with DPBS before use [27].
Subsequently, the solution was aspirated and the insoluble formazan product was solubilized with acidified iso-propanol.
The suspension was centrifuged at 1200 rpm for 6 min, and the sodium citrate solution was aspirated.
Further, the fixing solution was aspirated and traces of fixing agents were removed by rinsing several times with PBS.
For electrospinning, the fibroin solution was aspirated with a 2-ml syringe having a diameter of 0.55 mm.
Eventually, the staining solution was aspirated and traces of staining agent were removed by rinsing several times with PBS.
Similar(5)
After the solution is aspirated, the solid liquid contact electrification occurs at the interface between the pipet tip inner surface and the solution, thus resulting in the generation of electrical charge pairs into an electric double layer (EDL).
All media and fixation solution were aspirated, and cells were washed once with 200 µL warm PBS++.
Plates containing collagen coating solution were aspirated and air-dried in the laminar flow hood for 2 hrs before using them as collagen coated plates, whereas the plates containing concentrated solution were directly used for subsequent inoculation of 10T1/2 cells.
After that, the solutions were aspirated and washed again.
After that, the solutions were aspirated and each well was washed with 400 μl of Wash Buffer (R&D Systems).
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