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The remaining solution was amplified in isopropyl β-D-1-thiogalactopyranoside (IPTG -induced BLT5615 untIPTG -induced
For semi-quantitative PCR analysis, the cDNA solution was amplified for 45 cycles at an optimal annealing temperature.
After in situ reverse transcription, solution over the tissue section was recovered and the cDNA contained in this solution was amplified using standard liquid-phase PCR.
Next, 1.33 μl of cDNA solution was amplified using 10 μl of TaqMan 2 × Universal PCR Master Mix with no AmpErase UNG (Applied Biosystems), 1 μl of gene-specific primer/probe, and 7.67 μl of nuclease-free water in a final volume of 20 μl.
Then, 1.33 μl of the cDNA solution was amplified using 10 μl of TaqMan 2 × Universal PCR Master Mix with no AmpErase UNG (Applied Biosystems), 1 μl of gene-specific primers/probe and 7.67 μl of nuclease-free water in a final volume of 20 μl.
Next, 1.33 μl of cDNA solution was amplified using 10 μl of TaqMan 2 × Universal PCR Master Mix with no AmpErase UNG (Applied Biosystems), 1 μl of gene-specific primers/probe, and 7.67 μl of Nuclease-free water in a final volume of 20 μl.
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Moreover, the differences between two solutions are amplified when the observation positions are near the groundwater table.
Additionally, 1 µl of the proteinase K-treated cell solution used for sorting was amplified.
The blank (filtrated solution of precipitated Ag colloid) signal was amplified by 100 times.
The final linear deletion cassette was amplified from this solution by PCR.
Exon 9 was amplified using a PCR Enhancer Solution (Invitrogen) and MgSO4.
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