Exact(2)
Briefly, 4 μm sections of formalin-fixed, paraffin-embedded tissue were deparaffinized with Bond Dewax Solution (Vision BioSystems), and an antigen retrieval procedure was performed using Bond ER Solution (Vision BioSystems) for 30 minutes at 100°C.
In brief, cut sections were subjected to on-board dewaxing (Dewax solution; Vision BioSystems) and antigen retrieval (Epitope Retrieval 1 solution; Vision BioSystems) for 20 minutes before application of primary antibody (1 200) and detection by using the Bond polymer Refine detection system (Vision BioSystems).
Similar(58)
Helping farmers grow food in the face of extreme weather disasters would require new creative solutions, visions and partnerships.
The sector needs a joined up solution – our vision is to provide it.
Although his supporters point out that Lieberman – who lives in a settlement in the occupied West Bank – is open to a two-state solution, his vision includes the transfer of areas with Israeli Arab majorities out of Israel.
The central thesis is that attention acts to optimize the search procedure inherent in a solution to vision.
BT announced today that their IPTV solution, BT Vision will finally launch on 4th December which nicely coincides with the recent announcements about the rollout of BT's 21CN network in Cardiff.
Now, lvl5 thinks it has an even better, cheaper and more plentiful solution: computer vision software that extracts visual landmarks like stop signs and landlines, then aggregates the data into a kind of 3D map of the world that enables cars to triangulate their locations down to within an inch.
It's about them negotiating differing viewpoints to arrive at a common solution or vision that they can own together.
For antigen retrieval, slides were pre-treated with the Bond™ Epitope Retrieval Solution 2 (Vision BioSystems).
Tissue sections were retrieved using Bond Epitope Retrieval Solution 1 (Vision BioSystems) and stained with antibodies against hnRNP K (mouse monoclonal antibody, 1 300 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and MMP-12 (goat polyclonal antibody, 1 10 dilution; Santa Cruz Biotechnology).
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