At the end of the oxidation process, about 27 ml H2O2 was added into the solution to terminate the oxidation.
Following incubation at 37.5 °C for 20 min, 10 µL of p-nitrophenyl glucopyranoside (pNPG 10 mM) as substrate was added to the mixture to start the reaction and was then incubated at 37.5 °C for 30 min, followed by addition of 650 µL of 1 M Na2CO3 solution to terminate the reaction.
After 0.50 mg of protein (BSA, transferrin factor (TF), or IgG) was incubated with 15 μg of trypsin, 3.95 mg of ammonium bicarbonate, and 500 μL of deionized water for 24 h at 37 °C, 1.5 μL of 1.0 M hydrochloric acid was added into the solution to terminate the digestion reaction.
There is always the possibility to find a formally correct solution and to terminate.
It was used to raise the solution pH to terminate the Fenton reaction as well as to maintain pH before absorbance measurement.
The enzyme reaction was terminated by adding stop solution to each well.
Co-incubation was terminated by adding MTT solution to 0.5 mg/ml and reactions were allowed to stay for 3 more hours.
The reaction was conducted at 37°C for 2 h and was terminated by heating the solution to 70°C for 10 min.
Later, about 35%% hydrogen peroxide (H2O2) solution was added to terminate the oxidation process, and the color of the mixture changed to bright yellow, indicating a high oxidation level of graphite.
Stop solution was added to terminate the reaction.
