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Erythrocytes added to the solution cause the osmolarity of the solution to rise, and the difference in hydrostatic pressure to decrease, resulting in a prolonged time of cell lysis.
The PCDTBT solution infiltrates into the template by two wetting phenomena namely (i) capillary force (repulsion) which cause the solution to rise from the bottom of the template and (ii) with the assistance of gravity that contributes to the flow of solution from the top of the template into the nanopore (Figure 2a).
A strip (11 × 28 cm) of glass fibre paper (Whatmann GF/A) was inserted into a polyethylene bag (25 × 35 cm) as physical support for the development of the root and as a way for the nutrient solution to rise by capillary action and reach the whole root.
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CEO of Professional Science Editing for Scientists @ prosciediting.com