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Working standards were made by dilution of stock solution to final concentrations in urine.
The hormone was dissolved in ethanol (1 mg mL-1) and diluted in nutrient solution to final concentrations of 10-7 M and 0.5 × 10-9 M.
Aβ solutions were prepared by immediate dilution with 10 mM phosphate-buffered saline (100 mM NaCl, 0.5 mM EDTA, pH 7.4) to a concentration of 40 µg/ml and further diluted with CEppt solution to final concentration of 20 µg/ml (containing 5% (v/v) DMSO).
Additional clean up of the extended products was done by incubation with 1U SAP at 37°C for 1 hour and 75°C for 15 min; 0.5 µl of the purified SNP reaction to 0.5 µl size standard 80 (P/N 608395) and 39 µl of sample loading solution, to final volume of 40 µl for each in the CEQ sample plate (P/N 609801) and loaded.
All substances were stored as stocks (10 100 mM) at −20°C and diluted in Kint solution to final concentrations.
Antimicrobials in nanoparticle form and free in solution were prepared from the stock solution to final concentrations of 0.25X, 1X, 4X and 16X of the MIC previously determined.
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40 µg/ml Aβ40 or Aβ42 solution was immediately mixed with the cinnamon solutions to final concentration of 20 µg/ml Aβ and various cinnamon concentrations (0.01 µg/ml–100 µg/ml).
Calibration lines were prepared by diluting the appropriate stock solutions to final concentrations of 100, 50, 25, 10, 5, 2, and 1 pg/μL.
To explore the effects of pH on FIT, FIC, and FIT/FIC, we adjusted the pH values of the sample solutions to final pH values of 5.0, 6.0, 7.0, 8.0, and 9.0 and then tested the samples with a QB-ICA sensor.
Urea and dithiothreitol (DTT) were added from stock solutions to final concentrations of ~8 M and 5 mM, respectively and the samples were reduced and denatured at 60°C for 30 minutes.
Auschwitz was the final solution to the Final Solution.
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