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Cells were sequentially incubated with amiloride (10 µM, apical solution) to inhibit the epithelial sodium channel (ENaC), the cAMP-elevating agent forskolin (20 µM, apical and basolateral solution) to activate CFTR, and UTP (100 µM, apical solution) to trigger the activation of Ca2+-dependent Cl− channels [25], [26].
Anyone interested in using third-party TRIM with non-Apple-installed SSD drives with Snow Leopard and Lion can see Hardmac's [Updated] The universal solution to activate TRIM.
Herein, we reported a green and facile one-step process of electrochemical oxidation and expansion in salt solution to activate graphite foil (GF) for fabricating expanded graphite foil (EGF).
The template was first treated with a self-assembled monolayer (SAM) of octadecyltrichlorosilane (OTS) only on the protrusion surface using μCP, and then treated in SnCl2 solution to activate the surface of the trenches for the selective electroless plating (ELP) of silver inside the trenches.
To ensure that Ca2+ stores were depleted after thapsigargin treatment, we depolarized cells with high-K+ Ringer's solution to activate Ca2+-induced Ca2+-release.
Some experiments employed ADP-ribose (Sinma) in the internal solution to activate TRPM2 (transient receptor potential melastatin2) channels.
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Solutions to activate and relax fibers contained 5 mM free MgATP, 1 mM free Mg2+, 10 mM total EGTA, 25 mM total BES, pH 7, and when appropriate, 25 mM creatine phosphate and 15 units/mL creatine kinase.
In this model, called the recycling model, cortactin is hypothesized to prevent GST-VCA from being sequestered at branches, which could slow polymerization by limiting the concentration of GST-VCA in solution available to activate Arp2/3 complex.
Between tests in solutions expected to activate currents, the cilium was kept in an appropriate control bath for 2 min. Before testing for effects of ITP or ATP, the cilium was kept in a control bath containing the same nucleotide.
For total activity, 50 μL of 10 mM RuBP was added 15 min later, after 50 μL of sample extract was combined with 900 μL of an assay solution to fully activate all the Rubisco.
For total activity, 50 μL of 10 mM RuBP was added 15 min later, after 50 μL of sample extract was combined with 900 μL of assay solution to fully activate all the Rubisco.
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