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Experiments at pH 5.5 were performed in ND96 solution substituting 5 mM MES for HEPES.
Solution: Substituting Equation 131 in the left-hand side of Equation 132, we get I = − ∫ 0 a ( c 1 + c 2 t ) t ( a − t ) ( b − t ) ( c − t ) dt t − x + ∫ b c ( c 1 + c 2 t ) t ( a − t ) ( b − t ) ( c − t ) dt t − x.
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The placebo group received isotonic saline solution substituted for IB.
Pipette solutions substituting Cs+ ions for K+ ions are traditionally used to measure voltage-gated Ca2+ currents in the patch-clamp technique [11], [23].
Hence, the Er 3+-doped boehmite NP solution substitutes to the simple solution of Er 3+ ions used in the conventional preform soaking step.
Cells were then washed twice with PBS and incubated with TrypLE solution (substitute for porcine trypsin) for 5 min at 37°C to detach them from the flask.
It is known that a sub-optimal control can be derived from the classical LQG solution by substituting the linear filtering part with a quadratic optimal filter.
When the third leaf expanded fully, the seedlings were transferred into hydroponic systems (Hoagland nutrient solution which substituting 0.1 mM FeSO4 for 0.1 mM EDTA-Fe) and removed cotyledons.
For RNA extraction of minute tissue samples, tissues were once fixed with ethanol-acetate (3∶1) solution, and the solution subsequently substituted for PBS (Phosphate Buffered Saline) with 10% sucrose [16].
Subsequently the solution was substituted by either fresh Krebs solution or glucose-free Krebs solution (glucose substituted by equal moles of NaCl).
Unlike the above-mentioned methods, we use a variable substitution method to obtain a non-negative constraint of solution, in which the solution is substituted by a non-negative expression.
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