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Cells were then washed twice with PBS and incubated with TrypLE solution (substitute for porcine trypsin) for 5 min at 37°C to detach them from the flask.
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The placebo group received isotonic saline solution substituted for IB.
Hence, the Er 3+-doped boehmite NP solution substitutes to the simple solution of Er 3+ ions used in the conventional preform soaking step.
Experiments at pH 5.5 were performed in ND96 solution substituting 5 mM MES for HEPES.
Pipette solutions substituting Cs+ ions for K+ ions are traditionally used to measure voltage-gated Ca2+ currents in the patch-clamp technique [11], [23].
Random walk algorithm with compulsive evolution is a novel stochastic method with strong global search ability for heat exchanger network synthesis; however, its mutation behavior of accepting bad solutions might substitute excellent solutions with bad ones and consequently cost-optimal structures cannot be guaranteed.
Aiming at the exact solutions, we substitute a trial solution ξ ( x, y, z, t ) = λ ( y, z, t ) + e α ( t ) x + β ( y, z, t ), Open image in new window (21).
Two main objectives are present in this research work: (1) to study the possibility of exploiting local sand available in large quantities in a sand concrete and (2) to partially find a solution to substitute scarce coarse aggregate in concrete.
In order to determine linear stability of a bump solution U r), substitute u r, t) = U r) + p (r)e λ t into Eq. (1) and expand to first order in p using equation (4).
To a magnetically stirred solution of substituted quinazolinone (c1 c12, 0.01 mmol) in ethanol, substituted aldehyde (0.01 mmol) was added and the reaction mixture was stirred for 2 h.
Subsequently the solution was substituted by either fresh Krebs solution or glucose-free Krebs solution (glucose substituted by equal moles of NaCl).
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