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The non-linearity error (defined in the text) of the calibration curves (signal versus mass functions) with matrix-free solution standards was 30 70% without, and 1 5% with CCl4 vapor introduction, respectively, at 1 ng mass of Cu, Fe, Mn and Pb analytes.
Multielemental high-purity solution standards were used for the calibration.
Certified calibration solution standards purchased from Sigma Aldrich and NRC-CNRC (Certified Reference Materials Program, Institute for Marine Biosciences, National Research Council of Canada) were used for method development, analyte identification and quantitation.
Samples were pressed into pellets using paraffin and cellulose/ N-butyl-methacrylate. Standards were prepared by spiking synthetic ZnO/Fe2O3 matrix (1 1) with multi-element solution standards and Rh as an internal standard.
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Find the first level of approximate solution: standard multilevel method.
Find the second level of approximate solution: standard multilevel method.
Find the third level of approximate solution: standard multilevel method.
By making use of this stock solution, standard solutions of 20, 40, 60, 80 and 100 mg/l are prepared.
Hearts were removed and placed in cold (4°C) Krebs-Henseleit buffer solution (standard perfusion media).
From the stock solution, standard solutions with concentrations from 1 ng/L to 500 ng/L were prepared in an aqueous medium by dilution using Hamilton syringes.
All DOX in the supernatant was carefully collected and quantified based on the DOX (in PBS solution) standard curve.
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