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From this stock solution, serial dilutions were prepared using double distilled autoclaved water.
Starting from a chemical agent solution, serial dilutions were prepared in TSB inoculated with the test bacterial populations ≥ 10 CFU/mL.
Following an overnight cryoprotection in a 30% sucrose solution, serial 30-μm coronal sections of the brains were made with a cryostat microtome and collected in 0.02 M potassium phosphate saline buffer.
While the plates incubated with block solution, serial dilutions of the GST-FlpA, GST-FlpA-D1, GST-FlpA-D2 and GST-FlpA-D3 were made in PBS to produce concentrations that ranged from 1000 nM to 7.815 nM.
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First identify your weakness, then try one of the following solutions: Serial procrastinator Hire a serial nagger.
Other solutions used for the experiment are prepared from the stock solution using serial dilution.
The other solutions used for the experiment were prepared from the stock solution using serial dilution.
Fixed specimens were dehydrated in graded ethanol solution with serial concentrations of 50%, 70 %, 80 %, 90 and 100%.
Daily working standard solutions were prepared from the previously prepared working standard solution by serial dilutions with methanol to contain 1 - 10 μg/mL for benzophenone and to contain 200 μg/mL for phenytoin (final concentrations).
To generate high-speed motion with high-accuracy positioning, the parallel manipulator is a better solution than serial manipulator owing to its advantages such as high rigidity and positioning accuracy.
At the end of the experiment, all brains were fixed in a 4% formaldehyde solution and serial coronal slices were made on a microtome.
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