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Then we prove the global existence of solution (Section 3).
Finally, we prove the asymptotic behaviour of solution (Section 4).
The heuristic methods are presented in "Heuristic solution" section.
The suboptimal solution is presented in "Suboptimal solution" section.
The Table 2 represents the LCS grammar for the travel scenario discussed in "Solution" section.
In the 'Selection of compromise solution' section, selection criteria of compromise optimal solution are described.
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After staining with 2% uranyl acetate prepared in 50% ethanol and incubation with a lead citrate solution, sections were observed on a Hitachi H-600 transmission electron microscope.
After washing with phosphate-buffer saline solution, sections were incubated with the secondary antibody (Cy3-conjugated goat anti-rabbit IgG, or with fluorescein-congugated goat anti-mouse antibody, Chemicon International, 1/4000).
Following 10 min in aniline blue solution and 5 min rinse in acetic acid solution, sections were dehydrated and coverslipped.
After 10 min in Weigert's iron hematoxyline solution, sections were then dipped in Biebrich scarlet-acid fuchsin solution before differentiating in phosphomolybdic-phosphotungstic acid solution.
After the blocking solution, sections were incubated for 1 h ~O/N at RT with a rabbit anti-mouse type IV collagen Ab and washed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com