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In high-salt solution, secondary hairpin structure appears in ssDNA by the formation of base pairs between complementary bases, and external stretching causes a hairpin-coil structural transition, which is continuous for ssDNA made of random sequences.
After overnight incubation at 4°C with the anti-Mrp1 antibody (final concentration 1 µg/mL) in blocking solution, secondary anti-rabbit Alexa-conjugated antibodies (MolecularProbes, Carlsbad, CA, USA, 2 µg/mL, in 0.3% Triton-PBS) were added and incubated for 2 hr at room temperature.
The solution secondary structures of LGA11 and LS13 were then determined by enzymatic structure probing.
Antibiotic-antimycotic solution, secondary antibody (Alexa Fluor 488 conjugation), and phalloidin (Alexa Fluor 647) were received from Life Technologies (Paisley, UK).
That is, in TMAO solution secondary structure accretion, DSE contraction, and protein folding are driven by the unfavorable interaction with the peptide backbone, and these processes are opposed to a small extent by hydrophobic interactions.
After blocking with TNT Block TNTT + 10% NGS, 10% BSA) for 2 hours, samples were incubated overnight at 4°C in TNT secondary antibody solution (secondary antibody diluted 1 250 in TNT Block).
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Secondary antibody solution: Dilute secondary antibodies (anti-rabbit IgG-Cy3, anti-mouse IgG-FITC) at 1 200 in blocking solution.
We differentiate the methods of dynamic spectrum allocation as coordinated usage of resources, interworking solutions, integration solutions and secondary access of spectrum.
For multiuser uplink cognitive relaying schemes, in [8], authors have presented an optimal solution for secondary multiuser scheduling by selecting the optimal secondary source which maximizes the received SNR at the secondary destination.
Injection of slug of ionic solution in secondary mode provides lower recovery compared to that recovered with continuous ionic solution injection in the same mode.
As shown in the table, with the increase of the concentration of CA solution, the secondary pore volume of modified USY zeolite increases firstly and then decreases.
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