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The digest solution removed from the tube into an Eppendorf tube and the gel pieces were then washed with 50% acetonitrile (20 μl) for 10 min to recover more digest proteins from the gel.
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It was only when the restrictions became too strict, whereby all solutions near the optimum solution were removed from the solution space or records of selection decisions made at earlier selection times were removed, that we failed to realise most of the additional gain.
Samples were placed briefly on ice followed by centrifugation at 13,000 × g for 3 min. Blocking solution was removed from the hybridization chamber, and 145 µL of hybridization solution was applied to the arrays.
After 10 min, the solution was removed from the water bath, 1.2 mL of the seed solution added, and the mixture stirred for 2 min. The solution was then placed back in the 25 °C water bath for 18 h.
The solution was removed from the plate after cooling for 5 min. Once the solution reached room temperature, it was centrifuged until precipitation of the GNSs, and a clear suspension was obtained.
For preparing solution to electrospun pristine silk nanofibers, 20 ml of 8 wt.% of aqueous silk solution was removed from the refrigerator.
Next, gel precursor solution is removed from the well, thus leaving two empty wells and a channel filled with gel precursor solution.
After the SERS spectra were collected, the colloid and acidic solution were removed from the sample.
The autoclave solution was removed from the oven was allowed to cool down to room temperature.
Then, the solution was removed from the heat, and the stirring process continued for another 10 15 min.
The polymer solution was removed from the capillary tip faster as the jet ejected from Taylor cone.
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CEO of Professional Science Editing for Scientists @ prosciediting.com