It produced high quality results, improving several best solutions previously reported.
Ketamine was added to the perfusion solution, as previously reported (Freund et al. 2007).
Synthesis conditions were adjusted to achieve an average number of glycan molecules bound to the protein between 4 and 7 since maximum thermodynamic and colloidal stabilization effects in solution were previously reported for these constructs by Solá et al. [ 21- 24].
These values were used to estimate the water activity in these solutions as previously reported [31].
The conscious animals did not show any signs of discomfort during injection of the ALA-solution as previously reported by Edwards et al (1984).
Once a deep stage of anaesthesia was reached, mice were intratracheally instilled by means of MicroSprayer Aerosolizer system (MicroSprayer Aerosolizer- Model IA-1C and FMJ-250 High Pressure Syringe, Penn Century, USA) with 100 μg of PM1 in 100 μL of isotonic saline solution, or 100 μL of isotonic saline solution (sham) as previously reported [ 4, 10, 14].
The rate parameters of the sensing process were determined by combining the present transient-state model with the analytical solution of a previously reported steady-state model.
We have recast the canonical chemical engineering problem of batch reaction with fixed bed sorption into the form of a delay-differential equation, obtaining a more intuitive model and a simpler closed form solution than those previously reported.
According to enzymatic catalysis examination, the GOD/GNRs bioconjugates have extraordinary stability at high temperature in contrast not only with the free enzyme in solution but also previously reported GOD systems with other nanoparticles.
We conducted a performance-wise comparison of our solution, ChER, against previously reported machine learning-based chemical NER methods, namely, that of Corbett et al. [15], Rocktäschel et al. [16] and Nobata et al. [23].
