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The surface immobilization of the template vancomycin was performed by incubating the beads with a solution of the template in PBS, pH 7.2, overnight at 4°C (concentration of 5 mg mL-1).
Most of the procedures for chemical synthesis of MIPs involved noncovalent preassembly in solution of the template and a vinylic or acrylic functional monomer followed by bulk polymerization [ 59].
MIPs and nonimprinted polymers were weighted into polypropylene microtubes and the solution of the template (or analyte of interest) was pipetted on the particles in toluene or in acetonitrile.
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The immersion process has sanctioned the spreading of solution over the template's wall by creating an approximately 20 nm of wall thickness.
By this approach, optimum infiltration could be achieved through higher coverage of solution onto the template.
Since there exists an energy gradient between the solution and wall-surface of the template, hence, the solution spreads over the wall-surface of the nanochannels, thereby forming a thin layer of solution known as a precursor film [10, 31].
The algorithm dynamically compares the distances between the geometric objects of the student's solution and the template's solution, provided by the author of the exercise.
b Infiltration of ZnO precursor solution into the template and pre-baking several times, and c Post-baking for template removal.
The residual solution on the surface of the template was rinsed with distilled water.
The sensitization time was optimized to 5 min after a number of experiments, since prolonged dipping of AAO template in SnCl2 solution results in dissolution of the template.
The centrifuge tube was modified to optimize the infiltration process of the solution into the nanochannels of the template.
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