Sentence examples for solution of substrates from inspiring English sources

Preparation of stock and working solution of substrates, incubation conditions, and analytical parameters for assays.

At this point, the solution of substrate (nutrients) is fed into the reactor, without the removal of the culture fluids.

After 20 days of pre-culturing on a shaker (100 rpm) and 16 hours of photoperiod at 25 ± 1°C, a solution of substrate (100 mg in 1 mL of acetone) was added to each flask through a 0.2 μM membrane filter and the flasks were placed on a shaker for 10 days.

After 15 days of pre-culturing on a gyratory platform shaker at 100 rpm and with a 16 h photoperiod at 25 ±1°C, a solution of substrate (100 mg in 1 mL of acetone) was added to each flask through a 0.2 μM membrane filter and the flasks were placed on a shaker for 20 days.

Method B: Hydrogenation: Pd/C (10%%) was added to a solution of substrate in THF/MeOH (1 1).

Method D: Removal of the TIPS protecting group: Tetra- n-butylammonium fluoride (TBAF; 1 m in THF) was added to a solution of substrate in THF.

In addition, the transfected cells could be labeled and imaged a second time by incubating the sample with a fresh solution of substrate.

A stock solution of substrate (10 mg N-succinyl-LLVY-7-amido-4-methyl coumarin in 600  μl dimethyl sulphoxide) was diluted 1 : 1000 in 100 m M Tris/HCI, pH 8.0, for use.

Morpholine⋅BH3 (505 mg, 5 mmol), BBE solution (105 μL of a 354 μ m preparation; final conc. 0.05 g L−1) and a solution of substrate 1 e (150 mg, 0.5 mmol; final conc. 10 m m) in DMSO (5 mL) were added, and the mixture was shaken at 37 °C and 150 rpm.

Enzyme was dissolved in 50 mM Tricine buffer (containing 10 mM CaCl2 and 400 mM NaCl), pH 7.5, to give 0.8 units/mL (according to the supplier's activity data); a 2 mM solution of substrate FALGPA was prepared in the same buffer.

The preparation of stock and working solutions of substrates was shown in Additional file 1: Table S2.