Then, the solutions of bacterial spores in water yield clear evidence of biodetection in the statistical analysis of WGMs from 50 microspheres.
Figure 5c shows the values obtained from 50 microspheres in the 3.3 × 10−12 M solution of B. globigii spores.
The results are shown in Fig. 5d for both the 3.3 × 10−12 M solution of B. globigii spores and the control sample.
The black dots are for a 3.3E 12 M (1.9E9 spores/ml) solution of B. globigii spores while the open squares are for the control sample in DI water.
On the day of exposure, the medium was changed, a teaspoon of sterile sand and a solution of P. ramosa spores (treatments) or a sham solution (controls) were added to each jar.
A 2003 paper examined the efficacy and potential mechanism of action of a stabilized chlorine dioxide solution against spores of B. subtilis compared with hypochlorite solution (Young and Setlow 2003).
Most observations for wet solution had complete inactivation of spores, and could not be modeled using RSM.
We used data from Daphnia exposed to one of two doses (50,000 and 100,000 spores per Daphnia) of P. ramosa (the spore solution was a mix of spores collected from the same pond as the Daphnia, see Additional file 1 A1).
The spores were collected, washed twice then resuspended in 0.1% (v/v) Tween 20 in saline solution, and the concentration of spores was confirmed by haemocytometer counting and viable counting.
