Sentence examples for solution of protease from inspiring English sources

Chinchilla tissues were homogenized in 0.5 ml sterile saline with a 1 X solution of protease inhibitors (Roche, Indianapolis, IN).

MФs were infected as described above, washed with 4°C PBS, and incubated in a solution of organelle-specific 1× Extraction Buffer (Organelle Isolation Kits®, Sigma Aldrich) and a 0.1% solution of protease inhibitor cocktail (Sigma Aldrich) overnight (ON) on ice at 4°C.

Stock solutions of protease were serially diluted into a buffer comprising 10 mM HEPES (pH 7.5) and 500 mM NaCl as needed.

Bound bacteria were subsequently unbound by the addition of a 0.01% solution of protease/serine protease mix (Sigma) to each well, then the viable bacteria were quantified by plating serial dilutions onto TS agar plates.

A 25× solution of SIGMAFast protease inhibitor cocktail (Sigma-Aldrich Company, St . Louis MO) was prepared in digestion buffer; the cocktail was added to a final concentration of 1× following trypsin digestion.

*CC2 is a citrate based buffer, pH 6.0, Protease 3 is a low concentration solution of a serine protease and CC1 is a Tris buffer with 1 mM EDTA (antigen retrieval reagents, Ventana; 950-124).

To obtain NP cells from human IVD tissue, NP tissue was identified and dissected from AF. NP tissue was finely chopped and digested in a solution of 2 U/mL protease (Sigma-Aldrich, Gillingham, UK) in Dulbecco's modified Eagle's medium plus Ham's F-12 nutrient medium (DMEM + F-12) (Gibco BRL, now part of Invitrogen, Paisley, UK) for 30 minutes at 37°C.

Snap-frozen tissues were homogenized using a sonicator with PRO-PREP (Intron) extraction solution in the presence of protease inhibitors.

Snapped-frozen sperm were homogenized using a sonicator with PRO-PREP (iNtRON Biotechnology, Seoul, South Korea) extraction solution in the presence of protease inhibitors.

On the next morning additional 20 μl of protease solution was added and the incubation with shaking continued for 1 hour.

Embryos were then transferred into a 24 wells plate with 500 μl of Protease solution (0.25% trypsin, 1 mM EDTA, pH 8.0, sterile PBS) and 30 μl of Collagenase P (100 mg/ml, Roche).