Sentence examples for solution of native from inspiring English sources

A solution of native CysM−CysO was prepared by mixing separate 15 mg/mL solutions each of CysO and CysM in a 2 1 volumetric ratio.

A solution of native MoeZ−CysO was prepared by mixing separate 10 mg/mL solutions each of MoeZ and CysO in a 1 1 volumetric ratio.

Cleaved NS was obtained by incubating a 200 μM solution of native NS at 37 °C for 1 h with trypsin (Sigma), applying a 1 10 protease NS concentration ratio.

A 5 mg/mL solution of native starch was prepared in PBS and incubated at 37 °C with 4.5 nM (approximately 0.33 IU) porcine pancreatic α-amylase on an inclined rotating table to allow constant end-over-end mixing.

Latent and polymer NSs were produced by incubating a 20 μM solution of native NS at 55 °C for 2 h; the polymers were subsequently separated by size exclusion chromatography (SEC; Appendix A, Supporting Fig. A1).

Stock solution of native PIP2 (Sigma-Aldrich, P9763) was typically prepared at 1-5 mg/ml (1-5 mM) in water (MilliQ) and a series of concentrations was prepared using the same buffer as for EPR experiments (20 mM Tris-HCl 150 mM NaCl 1 mM EDTA pH 7.5 and 20% v/v glycerol).

For the molecular size-selective detection of a protein formulation, a mixture of solutions of native SOD1 (100 μg/ml) and its aggregates (the volumetric ratio between them was 1 1, 1 5, 1 10, 1 20).

Therefore, the 96-well ELISA was utilized to quantify unbound PSA at equilibrium in the solutions of native Abs (or Ab-PNIPAAm conjugates).

Thin films of fibrillar collagen were generated by adding neutralized solutions of native collagen solution (4°C, ≈0.4 mg/mL) to the alkanethiol-treated gold-coated coverslips and incubating overnight at 37°C.

These results were in qualitative agreement with the solution structure of native (ordered, folded) and denatured (disordered, unfolded) proteins, greatly differing in accessibility of aa residues (particularly hydrophobic aa's are usually buried in the interior of the native folded protein molecule) and consequently in the protein orientation and adsorption at the electrode surface.

The development of a brown colour correlated with the high intensity of free fluorescent products which was estimated to be 13-fold higher than the intensity of protein-bound fluorescent products and 41.5-fold higher than for the solution of the native protein (Fig.  1d).