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Next, the haustoria were transferred to a room temperature solution of lactic acid saturated with chloral hydrate for 5 d.
After bleaching, haustoria were successfully cleared in 1 3 d in a solution of lactic acid saturated with chloral hydrate at 42°C.
Once tissues became opaque white or translucent, they were washed three times with dH2O, then dehydrated in a graded ethanol series (minimum 2 h each: 50%, 70%, 95%, 3× 100%), and placed in a solution of lactic acid saturated with chloral hydrate at 42°C until cleared (3 d).
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Thereafter, the roots were immersed at 80°C for 1.5 h in the staining solution consisting of lactic acid:glycerol:water (1 1 1 v:v v) and 0.1% of each Trypan Blue and Methylene Blue.
For this, the control and treated leaves were boiled for 1 min in a freshly prepared solution of phenol, lactic acid, glycerol and distilled water (1 1 1 1) containing 20 mg/ml Evans blue.
Evans blue staining to assess cell death was carried out by gentle heating the FA-treated and control leaves for 1 min in a freshly prepared solution of phenol, lactic acid, glycerol and distilled water (1 1 1 1) containing 20 mg/ml Evans blue.
For quantitative assessment, inoculated leaves were treated with ethanol 100% for two hours at 65°C and 90% relative humidity before staining with trypan blue solution (25 µg/ml trypan blue in a 1∶1∶1 solution of glycerol, lactic acid and water,) for 15 minutes.
A biocompatible porous scaffold obtained via electrospinning a nanocomposite solution of poly l-lactic acid) and 4-methoxypoly l-lacticonacided multi-wanded carbon nanotubes is presented here for the first time for the enhancement of neurite outgrowth.
Another important recent advance is the aqueous solution of citric acid, lactic acid, hydrogen peroxide and a proprietary hydrogen peroxide stabilizer (to slow the decomposition of hydrogen peroxide to water and oxygen gas, Eq. 1), comprising a produce wash (First Step + 10), whose antimicrobial effect is due to the formation of perorganic acids (Eq. 2) [37].
The stained leaves were then fixed with a solution of 3 1 1 ethanol: lactic acid: glycerol and photographed.
CS PLA complex, which are well dispersed and stable in aqueous solution, was prepared by the precipitation of lactic acid in chitosan solution by dropping method and characterized by SEM, TEM, DLS and FTIR.
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