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The mice were anesthetized by injecting 40 μL of a 9 1 solution of ketamine (100 mg/mL) and rompun (100 mg/mL).
The mice were anesthetized with a solution of ketamine, xylazine, and saline (0.07 ml i.p).
In preparation for the irradiation procedure, mice were anesthetized in groups of four at a time with a combination solution of Ketamine and Xylazine as described previously [48].
Animals were lightly anesthetized with a solution of ketamine (Ketavet; Gellini, Aprilia LT, Italy) and xylazine (Rompun; Bayer, Wuppertal, Germany) before inserting the nose plugs (polyethylene tubing, 0.7 mm) into the right naris for 42 days [42].
At the end of the survival time, mice were deeply anesthetized with an intraperitoneal injection of a solution of ketamine (Ketavet; Gellini, Aprilia LT, Italy) and xylazine (Rompun; Bayer, Wuppertal, Germany) and perfused transcardially with 0.9% saline, followed by 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
After a protocol-directed period of environmental acclimation, induction of anesthesia was attained on day 0 by intramuscular injection of 0.1 mL of a solution of ketamine hydrochloride 100 mg/mL, xylazine hydrochloride 30 mg/mL and acepromazine 5 mg/mL.
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Orthotopic implantation was performed with the animals anesthetized by intramuscular injection of 0.02 mL of a solution of 50% ketamine, 38% xylazine and 12% acepromazine maleate.
Skull images were obtained after the animals were anesthetized with an intramuscular injection of a solution of 10% ketamine and 2% xylazine hydrochloride at doses of 30 and 10 mg/kg, respectively.
Mice were anesthetized by an intraperitoneal injection of a freshly prepared solution of 75 mg/kg ketamine (Pfizer) and 10 mg/kg xylazine (Calier).
To minimize unnecessary suffering, before gene delivery into tibialis muscles, the mice were anesthetized by an intra-peritoneal injection of a solution of 80 to100 mg/kg ketamine (Intravet, Italy) +10 mg/kg xylazine (Sigma-Aldrich, St . Louis MO, USA).
Briefly, mice were anesthetized i.m. with 40 µl of a solution containing 57% of ketamine (Dopalen, Vetbrands, Brazil) and 43% of xylazine (Dopaser, Laboratório Calier do Brasil LTDA, Brazil); after approximately 10 min, their necks were hyper-extended, and the trachea was exposed at the level of the thyroid and injected with 3×105 yeast cells in 50 µl of PBS using a 30-gauge needle.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com