Sentence examples for solution of enzyme from inspiring English sources

The enzyme-inhibitor constant Ki can be measured directly by various methods; one extremely accurate method is isothermal titration calorimetry, in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Thermostability of the enzyme was estimated by incubating the diluted solution of enzyme at different temperatures (75°C, 80°C, 82°C and 85°C).

The mass of precipitates obtained corresponds to the mass of precipitable protein (PP) present in 1 mL of the commercial solution of enzymes.

In cases where it was important to keep all cell surface proteins intact, a solution of enzyme-free 0.2 g/l EDTA4Na and 0.2 g/l glucose in PBS was used to dissociate the cells.

Tissue obtained from metastatic lesions was mechanically fragmented and incubated for 2 16 h in a solution of enzymes containing collagenase (type IV 0.1%), hyaluronidase (type V 0.01%) and DNase (type I 0.00007%).

Seven serially diluted solutions of enzyme digested plasmid DNA (with UV estimated concentration from 106 to 1 copies/µL) were used to establish the PCR calibration curve.

However, addition of BSA to solutions of enzyme also resulted in a large increase in the β-glucosidase activity for Cellic® CTec2 in the experiment without addition of lignin.

Equimolar complexes of DHFR.TMP, DHFR.NADPH, DHFR.folinic acid, DHFR.TMP.NADPH, and DHFR.folinic acid.NADPH were prepared by adding excess ligands to 1−4 mM solutions of enzyme in 50 mM potassium phosphate and 100 mM KCl, pH* = 6.5 (the pH* values being meter readings, unadjusted for deuterium isotope effects).

In reference solution, instead of enzyme, 20% BSA was used to hold the protein concentration constant.

Isolated cardiomyocytes were then transferred to the same solution, free of enzyme, containing 1 m m CaCl2.

Due to the complexity of commercial solutions of enzymes, it is possible that equal masses of lipase/protein are found in different Units or volumes of the commercial solutions.