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Ott first took a rat kidney and stripped out its functional cells using a solution of detergent.
Next, the proteins and fats that make up the cellular and nuclear partitions are broken down and removed with a solution of detergent.
Upon dilution of aqueous mixed micellar solution of detergent and phospholipids with buffer, the micellar size and the polydispersity increase fundamentally, and as the system is diluted beyond the mixed micellar phase boundary, a spontaneous transition from polydispersed micelles to vesicles occurs.
Samples were centrifuged and digested in a solution of detergent (sodium dodecylsulfate) and proteinase K, for removal of proteins bound to the DNA.
Sample equipment was cleaned in a 1% solution of detergent and hot water between sample collection to prevent cross contamination.
A 1% (w/v) solution of detergent was prepared, and the substrates were first cleaned by sonicating them in this solution for 10 minutes using an ultrasonic bath.
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Firstly, samples were cleaned in solutions of detergent, H2SO4/H2O (1:4), NH4OH/H2O2/H2O (1:1:5), and HCl/H2O2/H2O (1:1:6), and treated with HF/H2O (1:50) solution, followed by rinsing in deionized water to remove native oxide at the surface.
Lipase solution without addition of detergent solution was used as positive control while buffer without any lipase and detergent solution was used as negative control.
In this work, molecular dynamics simulations have been used to investigate the structural stability of a representative porin, OmpF, in three different environments: (i) aqueous solution at pH = 7; (ii) a solution of neutral detergent in a concentration similar to the critical micelle concentration; and (iii) the protein embedded into a neutral detergent bilayer.
In a typical experiment, 0.4 mM KAuCl4 was added to 10 mL of 1 wt.% aqueous solution of the detergent at room temperature, leading to the slow formation of AuNPs, as manifested by a pinkish coloration of the solution.
The coverslips were cleaned in a boiling 1 4 solution of 7× detergent (MP Biomedicals, Solon, OH) and purified water.
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