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After further rinsing and drying, 200 mg acid-purified nanotubes and 50 mg Fe3O4 nanoparticles were dispersed in 40 mL solution of deionized water/ethanol (1 1, volume ratio).
Using an anion-selective (but proton permeable) membrane to separate the impure acid solution from a solution of deionized water creates a sulfate concentration gradient and anion movement across the membrane.
The cleaned silicon wafers were immediately immersed in a mixed solution of deionized water, HF (4.6 mol/L) and AgNO3 (0.02 mol/L) for 3 min to grow nanostructures.
Chemical etching was followed in a mixed solution of deionized water, HF (4.8 M), and H2O2 (0.5 M) to form a vertically aligned SiNW array with a diameter of 60 ± 33 nm.
Then, the FTO was put into the autoclave containing a mixed solution of deionized water (20 ml), hydrochloric acid (20 ml), and titanium isopropoxide (1.1 ml) and baked at 160 °C for 6 h.
The FTO substrates were ultrasonically cleaned for 10 min in a mixed solution of deionized water, acetone, and 2-propanol with volume ratios of 1 1 1 and were placed at an angle against the Teflon container wall with the conducting side facing down.
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The FTO-coated glass was ultrasonically cleaned by detergent, saturation (CH3 2CHOH solution of NaOH, deionized water, and ethanol.
Proteins loaded into the column were washed with a linear gradient from 1 M of ammonium sulfate water solution to deionized water at an elution rate of 5 mL/min for 40 min followed by an isocratic elution with deionized water for 10 min at the same elution rate.
The solution was centrifuged with a suitable amount (about 200 to 300 mL) of deionized (DI) water added under an iced bath temperature.
To our knowledge, this is the first report to show that the emission wavelength and PL intensity of CDs can be easily tuned by adjusting the concentration of CD solution with deionized water.
A calibration curve was established based on six concentrations of 4-AP standard solutions in the range of 0.0125 to 0.2 mM by serial dilution of the standard stock solution with deionized water.
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