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NaOH (6 N) was added to a stirred solution of crude 3,4-bis(benzyloxy phenyl formate in MeOH (15 mL).
The 1% stock solution of crude extracts of bark, leaf, fruit pulp and seed were prepared with methanol.
Alternatively, a model solution of crude glycerol, which consisted of 160 mM glycerol and 770 mM methanol, was used as a substrate and fed to the reaction mixture in the same manner.
In order to assess the compatibility of the in vitro system with crude glycerol, a model solution of crude glycerol consisting of 30% (w/v) glycerol and 50% (w/v) methanol was prepared and used as a substrate for lactate production.
Briefly, cells were dissociated from minced hearts of 1- to 3-day neonatal Wistar rats with a 0.25% solution of crude trypsin.
Adding a solution of crude 16 to a solution of alcohol 17, nBu4NI, Hünig's base and 4 Å molecular sieves in CH2Cl2 successfully effected glycosylation.
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The aim of this paper is to provide new data on the properties of molecular aggregation in toluene solutions of crude oils and of solid asphaltenes.
Solutions of crude extracts and subfractions were prepared each of 2 μg/ μL concentration.
Stock solutions of crude extracts and fractions were added into respective wells.
Stock solutions of crude extracts and the positive control, ascorbic acid (400 μg/mL), were prepared in methanol at appropriate concentrations and added to DPPH (200 μL at 100 μM prepared in methanol) in a 96-microtitre plate.
To a solution of the crude ornithine derivative in DMF, guanidinylation reagent 31, NEt3 and AgOTf were added.
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