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The prepared solution of collagen (5 wt%) in HFIP was stirred magnetically for overnight.
The solution of PLGA was kept stirred overnight similar to nHA-GA dispersed solution of collagen.
The 5 wt% solution of collagen in HFIP at a flow rate of 0.5 mL/h without nHA and with 5 wt% nHA has produced nanofibrous meshes.
The multilayered scaffolds fabricated using 5 wt% solution of collagen with 5 wt% nHA (Fig. 7b) were found to be more bioactive.
To prepare the solution of collagen-containing nHA-GA, 0.03 g of nHA-GA was sonicated in 5 mL solution of HFIP for about 10 h before dropwise mixing to 5 mL of 10 wt% solution of collagen in HFIP under stirring.
Silk fibroin scaffolds were prepared by freeze-drying before immerging in a solution of collagen, decellularized pulp, and collagen/decellularized pulp.
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To examine the potential formation of functional vascular networks in vivo, a liquid pre-polymer solution of collagen-Ph containing human ECFCs and MSCs, horseradish peroxidase and hydrogen peroxide was injected into the subcutaneous space or abdominal muscle defect of an immunodeficient mouse before gelation, to form a 3D cell-laden polymerized construct.
Collagen fibrils can assemble spontaneously from purified solutions of collagen molecules.
To test this hypothesis, we therefore modified poly(dl-lactic-co-glycolic acid) (PLGA) substrate by coating it with natural biomaterial solutions of collagen, chitosan, or N-succinyl-chitosan, and then used these three combinations as scaffolds to evaluate their effects on osteoblast attachment, proliferation, and differentiation.
In previous experiments, we found that peptide II-1 inhibited fibrillogenesis of diluted solutions of collagen types I and II, whereas peptide II-44 lacked this effect [ 36].
Collagen and silk proteins were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Fisher Scientific, Pittsburgh, PA) separately, and the solutions of collagen and silk protein were mixed to make solutions containing silk at 0% (pure collagen), 15% (CS15), 30% (CS30), 60% (CS60), and 100% (pure silk).
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