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To examine the antifouling property, it was recorded two cyclic voltammograms in solution of buffer (pH = 7.0) and IP at a scan rate of 10 mVs−10
The conditions of analysis are as follows: 4.6 mm × 25 cm column, temperature 60 °C, mobile phase A; 1.80 g/l solution of buffer phosphate adjusted to pH 8.9, mobile phase B; methanol:acetonitrile (250 750 V/V), flow rate 1.0 ml/min, detection spectrophotometer at 210 nm and injection volume 50 μl.
A 100 μL solution of Buffer II (50 mM Tris HCl, 20 mM EDTA, 0.35 M SDS, pH 7.4) was added and the cell suspension was incubated at 65 °C for 5 min and then 80 μL of 5 M potassium acetate was added following incubation at − 20 °C for 5 10 min.
Cells were blocked and permeabilized for 45 min with a solution of buffer A containing 5% (v/v) NGS (normal goat serum), 50 mM NH4Cl and 0.5% saponin.
Variable-pH experiments were performed by changing the pH of a master solution of buffer using HCl or NaOH, taking small aliquots at a given pH, purging the buffer with argon, and then bringing the samples into the inert chamber.
For each adsorption isotherm at given pH and salt concentration, a stock solution of buffer (10 mM formiate, MES, BICINE, or CAPS) was freshly prepared and adjusted to the desired pH with aqueous HCl or NaOH solution (1 M).
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Tissues were placed in a 10% solution of buffered formalin.
Principal vital organs (liver, kidney, lung, heart, and spleen) were preserved in a fixation medium of 10% solution of buffered formalin for histopathological study.
Principal vital organs (liver, kidney, lung, heart, and spleen) were preserved in fixation medium of 10% solution of buffered formalin for histopathological study.
Tissue samples were fixed in 10% (v/v) solution of buffered formalin for 24 h at 4°C, then dehydrated, cleared in xylenes, and embedded in paraffin.
Samples for any given experiment were prepared by mixing stock solutions of buffer, MVox, dithionite, BNN, Cat, and Mb in a 1.5 mL microcentrifuge tube, to give a total volume of 150 μL.
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