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After washing with 750 µL TPB buffer to remove any unbound DNA, a 10 pM solution of beads were introduced into the chamber and incubated for 20 minutes.
After a brief centrifugation, plates were incubated at RT for 30 min. A 2× solution of beads was made such the final concentrations of both the acceptor and donor beads were 25 μg mL 1.
A stock solution of beads, with initial concentration of 2.1 × 10 8 particles/ml, was diluted into DI water and the concentration of beads was adjusted to 200 : 1 that produced sufficient bead density for imaging multiple beads in the observed volume.
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This was achieved by dispersing the surface-modified UCNPs in a water solution of bead precursor prior to the droplet formation.
Second we filled a glass capillary of known thickness (10 μm±10%) with a solution of fluorescent beads (Ø 0.5 μm, Latex beads, Sigma) and measured the geometrical path length, by the use of a calibrated piezo element, calculating the correction factor nk on the basis of the difference of the capillary and geometrical length:.
At t = 10 min the solution of the beads was rinsed out of the measurement chamber for 20 minutes and the flow stopped again.
A solution of silica beads (micromod sicastar-greenF 40-02-403) was minto into 1% LMP agarose.
CGWS has been validated in a solution of scattering beads [ 10, 12] and up to a depth of 200 µm in the weakly scattering zebrafish forebrain [ 11].
Initially, spraying of solution and formation of beads occurred while electrospinning from the silk fibroin solution.
A third group of bees was anesthetized and injected with 8 μl of a CM-25 Sephadex beads (Sigma-Aldrich, Steinheim, Germany) solution (0.01 g of beads mixed with 500 μl sterile bee saline and vortexed, resulting in approximately 110 beads/8 μl).
The functionality of the sensor filter was examined using a special designed measurement chamber with solutions of polystyrene beads and yeast (Saccharomyces cerevisiae).
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