Sentence examples similar to solution of apo from inspiring English sources

The center field of the EPR scans was set to 3,500 G with a sweep width of 1,000 G. EPR samples were prepared by titrating Mn2+ into a solution of apo-SsTroA.

A solution of apo-transferrin sample 2 (3 μ m in 3 m m NaHCO3) was incubated at 37 °C for 24 h.

Immediately after Fe2 ALC)3 was mixed with a solution of apo-FbpABp the λmax shifted from 428 to 410 nm.

The affinities (Kd; eq 3) of all the holo-siderophores for FbpABp were calculated using these concentration dependent quenching data as described in Experimental Methods. Figure 3a shows representative quenching spectra when Fe2 ALC)3 was titrated into a solution of apo-FbpABp, and Figure 3b shows the fit of these data using a one site binding model.

Aliquots of hemin (1.3 μL) solubilized in 0.1 M NaOH and diluted in 50 mM Tris (pH 8.0) and 100 mM NaCl were added to 1 mL of a 5 μM solution of apo-IsdB-N2 or a reference cuvette containing buffer alone.

As a summary, we have determined the solution structure of apo GrxS14, and investigated the structures of the apo GrxS14 dimer, its complex with GSH, and holo GrxS14.

The solution structure of apo F1 CTnC was determined by standard high-resolution NMR methods and an automatic iterative procedure that makes use of Aria (21).

In this work we report the NMR solution structures of apo lc DHFR and its DHFR.NADPH binary complex and compare these with our previously determined structures of the DHFR.TMP binary(12) and DHFR.TMP.NADPH ternary(10) complexes.

We have used NMR spectroscopy to determine the solution structures of apo lc DHFR and its binary complex with NADPH and TMP(12) (PDB ID: 2L28, PDB ID: 2HQP, and PDB ID: 2HM9).

Solutions of apo-HasAp variants in 200 mM HEPES buffer (pH 7.0) were prepared, and their concentrations were calculated as previously described on the basis of a 280 nm molar extinction coefficient (ε280) of 27.13 mM 1 cm–1 for both variants.

Structure refinement: The initial model from the molecular replacement solution of the apo structure was further used as input for the automated chain-tracing/model building program PHENIX AutoBuild.