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"I place this in a solution of acids for one day, then heat the solution on a hot plate".
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As a result, a black colored solution of acid-functionalized graphene was obtained.
For the experiment, a set of beakers with immersion capacity of 250 ml were filled with 100 ml test solution of acid 1N HCl, H2SO4, and HNO3 concentration.
In a typical synthesis, 0.45 M TNB and 0.90 M solution of acid were added to n-hexane in a total volume of 26 ml.
In this research, the aqueous solution of Acid Red 274 (AR 274) was selected as a model for textile wastewaters for evaluation under WAO conditions.
A stock solution of acid 6 (30 mM) was prepared in EtOH.
Briefly, purified type I collagen was purchased as a solution of acid-stabilized monomer (Vitrogen; Cohesion Technologies, Inc., Palo Alto, CA).
ESI-MS calcd for [C24H37O3]−, 373.27; observed, 373.37 A stock solution of acid 5 (7.5 mg, 20 μmol) was prepared in 2 mL of EtOH.
A mass spectrum resulting from mixing a solution of acid-denatured holo-Mb (pH = 2.9; Figure 4a) and a solution of 100 mM aqueous ammonium acetate at a 1 1 ratio is shown in Figure 4b.
The desired enone acid (1.0 equiv) and iPr2NEt (1.1 equiv) were dissolved in CH2Cl2 (to give 0.2 m solution of acid) in a flame-dried round-bottomed flask and cooled to 0 °C before tBuCOCl (1.2 equiv) was added dropwise.
The desired enone acid (1.0 equiv) and iPr2NEt (1.5 equiv) were dissolved in CH2Cl2 (to give 0.2 m solution of acid) in a flame-dried round-bottomed flask before tBuCOCl (2.0 equiv) was added dropwise.
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