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Pairs of mouse hippocampi or similar sized sections of cerebral cortex, were minced with micro-scissors and transferred to loosely-capped universal tubes containing 1 ml solution of a solution of 20 units/ml papain in 1 mM l-cysteine and 0.5 mM EDTA and 0.005% DNAse (Worthington).
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The priming solution consisted of a crystalloid solution and mannitol (3 ml/kg).
Fullerene-framework films were synthesized by adding HCl (6 μL of a 0.2 m solution) to a solution of Pluronic F127 (12 mg, 0.95 μmol) in ethanol (200 μL).
Brushite, CaHPO4·2H2O, was synthesized via precipitation by stoichiometric addition of an ammoniated calcium nitrate solution into a solution of ammonium dihydrogenphosphate, followed by a 2-hour maturation stage.
The cell lysis solution was a solution of 5% (w/v) sulfosalicylic acid in deionized water containing 0.1% (v/v) Triton X-100.
Finally, in Section 4, we discuss the convergence of these solutions to a solution of unperturbed problem as (c rightarrow0).
Moreover, we discuss the convergence of these solutions to a solution of the unperturbed problem as (c rightarrow0).
The samples were covered with 1 ml matrix solution (a saturated solution of a-cyano-4-hydroxycinnamic acid in 50%% acetonitrile, 2.5 % trifluoroacetic acid).
Before staining, 5 µl of 30% H202 was added to 1 ml of a stock solution of benzidine solution.
Zebrafish embryos were dechorionated at 24 28 hpf and transferred to a solution of E3 solution containing 1 mM PMSF.
This method produces sequential populations of alternative solutions, until a solution is found with satisfactory results.
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