Sentence examples for solution life from inspiring English sources

Briefly, they were rinsed with D-Hank's solution (Life technologies, Bangkok, Thailand) twice, and delivered into centrifuge tubes with a plastic scraper, followed by centrifugation at 2000 rpm for 15 min, with the supernatant removed.

Femora and tibia were collected from mice, and the bones were suspended in wash medium containing RPMI 1640 (Hyclone), 10% FBS (Hyclone), antibiotic/antimycotic solution (Life Technologies, Grand Island, NY), and HEPES buffer (Fisher Scientific, Pittsburgh, PA).

Isolated RNA was resuspended in RNA storage solution (Life Technologies).

Cell death was then quantified by trypan blue staining solution (Life Technologies, CA, USA).

Samples were preserved in RNAlater solution (Life Technologies) and stored at −80° prior to RNA extraction.

Cells were treated with EDTA solution (Life Technologies) and harvested upon reaching 80% confluence.

Hank's balanced salt solution (Life Technologies, 14025-092) was used for nutrient-deprivation studies.

Total RNA was extracted using TRI-reagent solution (Life Technologies) according to the manufacturer's protocol.

These samples were stored at –20 °C in RNAlater solution (Life Technologies) until processed for DNA or RNA extraction.

HEK293 cells were gently detached from plates after short incubations (60″) in 1X Trypsin-EDTA solution (Life Technologies).

Precipitated RNAs were suspended in 10 uL Urea Loading solution (Life Sciences) and incubated for 5 minutes at 95 °C.