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For oral rehydration the solution is made by using oral rehydration salts (ORS)—a measured mixture of glucose, sodium chloride, potassium chloride, and trisodium citrate.
A binder solution is made by mixing 2.0 gm of polyvinylidene fluoride (PVDF) polymer in 10.0 ml of dimethyl formamide (DMF) solvent.
The 15 wt % benzoic acid in decanol solution is made by stirring weighted benzoic acid powder in decanol (Sigma-Aldrich) until all solid are dissolved.
The 6.2 wt % Na2HPO4 (Sigma-Aldrich) solution is made by stirring weighted Na2HPO4 powder in deionized water until all solids are dissolved.
The glycogen stock solution is made by diluting Ultrapure Glycogen (Invitrogen) with nuclease-free water (Molecular Grade Water, Cat. No. E476-5, MidWeScientificfic) and 19.4 μl of a 1.4274 μg/μl glycogen solution is added to each sample.
The solution is made by diluting one vial of triamcinolone acetonide (40 mg) with 100 ml normal saline, which is 1/100 of the usual concentration used in steroid injection therapy.
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The ChTX solution was made by adding 1 mM ChTX (Sigma-Aldrich, Art. No. C7802) to the extracellular solution.
The sodium chloride solution was made by dissolving pure sodium chloride in distilled water.
50 mM BH4 −/OH− solution was made by dissolving NaBH4 with the same molar amount of NaOH in aqueous.
Control solution was made by adding 5 ml of DMSO in 10 ml of sterilized distilled water.
The working solution was made by mixing the two stock solutions in equal quantities and allowed them to react for 12 h.
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CEO of Professional Science Editing for Scientists @ prosciediting.com