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The naked clay powder was then refluxed with tetra(4-pyridyl)porphyrinatomanganese(III) 4-pyridyl porphyrinatomanganese IIIwion continuous stirring for different tiMnTPyP+
For this purpose, a N2-saturated hydroquinone aqueous stock solution was prepared by mixing a N2-saturated PHQ solution in methanol with solid NaBH4.
Reactions were initiated by addition of 8 μL of azide (from a 0.5 M stock solution in methanol) with a syringe, and the reaction mixture was stirred for 16 h at room temperature, under positive argon pressure.
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A work solution (1 ppm EB) was prepared by diluting 10 ml of 100 ppm stock solution (EB in methanol) with 990 ml of seawater.
Samples of peptides (1 μl) deposited and dried on target were overlaid with 1 μl of diluted matrix α-cyano-4-hydroxy-cinnamic acid (one volume of saturated solution in methanol mixed with two volumes of 50% methanol and 0.3% trifluoroacetic acid (TFA)).
An aliquot of 0.5 ml of sample solution in methanol was mixed with 2.5 ml of 0.5 mM methanolic solution of DPPH.
An IS stock solution in methanol was diluted with 50% aqueous methanol to obtain a 200 ng/mL working IS solution before storage at 4 °C.
The reaction mixtures, containing 120 μL of 0.04 mg/mL DPPH solution in methanol, were mixed with 20 μL of different concentrations (25 400 μg/mL) of the extracts and shaken vigorously before being incubated in the dark for 20 min.
The title compound was prepared by reaction of a solution of thiosemicarbazide (15 mmol) in methanol with a solution of HCl (30 cm3, 2 M), 1 cm3 of concentrated HCl and a solution of benzyl (15 mmol) in methanol at room temperature to form 5-Methoxy-5,6-diphenyl-4,5-dihydro-2H-1,2,4-triazine-3-thione [6].
Endogenous peroxidase activity was blocked with 3%H2O22 solution in methanol.
Endogenous peroxidase activity was blocked with H2O2 solution in methanol (0.01 M), for 30 min.
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