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SF soluble fraction, IF insoluble fraction, C cells, M medium, P1 periplasm fraction (hypertonic solution fraction), P2 periplasm fraction (hypotonic solution fraction), S spheroplasts.
Figure 3 shows the distribution of A. ferrooxidans density between the surface bound and solution fraction as a function of time for the bacteria/lipid/pyrite system.
The AgNO3 treatments generated higher Ag concentrations in the <0.45 μm solution fraction than did an equivalent treatment of nAg (i.e. spiking to similar total soil Ag levels).
The muscle homogenate is filtered through gaze and diluted 5 times with 0.25 M Sucrose solution (fraction 1, whole muscle homogenate-I).
One part, corresponding to B = 1, maps to the beads while the other part has a value of B > 1, and it maps to the solution fraction.
Each solution fraction was diluted by 1640 medium to an equal concentration of 5 μg/ml (herb weight per solution volume), which was used to induce CLP-stimulated sepsis.
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The pooled sample solution was then subjected to IEF using a ZOOM® IEF Fractionator (Invitrogen), and solution fractions with radioactivity were recovered, concentrated with a Centricon apparatus and subjected to analytical 2DE.
A total of 3660 different designs, with different solution fractions and working parameters, were analyzed by means of energy-exergy and net present value (NPV) analysis.
Elution of gelatinases was accomplished by using a 10% DMSO solution; fractions were then dialysed against MilliQ water for lyophilisation and Western blotting analysis.
Since coumarin, rutin and hyperoside were reported as components of the melilotus plant that were anti-inflammatory [ 8], they were used as standard substances to detect the effects of different solution fractions of ethanol extract from M. suaveolens Ledeb.
For acetone solutions (Fraction C, C1 C4, and α-T), 0.5 ml of each solution was pipetted onto the Whatman #1 filter paper lined in the Petri dish.
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