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The solution experiments were complemented with molecular dynamics simulations to elucidate the microscopic origins of the stabilizing effect of the consensus substitutions, which can be traced to the fast conformational dynamics of the folded ensemble.
In this study, water soluble, acidic alkaline and carbonated solution experiments were designed, and mineral reaction mechanisms were researched using chemical kinetics and the minimum free-energy method.
Water soluble, acidic alkaline, carbonated solution experiments were designed, and studied the influences of pH and CO2 on the formation mechanism of metasilicate mineral water through the simulation test in door.
Peptides used for the in solution experiments were commercially purchased from Intavis AG (Köln, Germany).
The pH of the solution was raised to approximately 8 using a sodium hydroxide solution; experiments were performed using solutions to which base was not added, these having a pH in the range of 4 5.
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However, comparisons of the results obtained from these experiments to those from bulk-solution experiments are complicated because of the dynamic nature of ESI.
Coke solution loss experiments were implemented by using a Cahn Thermax 700 thermogravimetric analyzer.
Differential, solution HDX experiments were performed at 4 °C.
Solution scattering experiments were performed at 293 K on a SAXSess mc platform (Anton Paar, Austria) equipped with a sealed tube source and a CMOS diode array detector.
As described above, molecular dynamic simulations predict that the interaction between the first part of IgFLNa20 and the CD face of IgFLNa21 is stable, but to test this in solution NMR experiments were performed.
Fluoride solutions for batch experiments were prepared from stock solution by appropriate dilutions.
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