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Cells washed with HANKS balanced solution, counted and cultured into 24 well plates at 4 × 105 cells per mL.
The results were listed as a percentage of the injected activity per gram of tissue mass (% IA/g), using counts of a defined volume of the original injection solution counted at the same time.
The metrics used measured the methods and classes of the solution, counting the number of rejected, duplicate and unused methods as well as the number of featureless or abstract classes.
The suspension was centrifuged and an aliquot of the supernatant solution counted on a scintillation counter.
After washing the leukocytes were resuspended in the same solution, counted and adjusted to a final concentration of 106 cells/mL.
In 3 5 day intervals, the cell monolayers were disrupted with trypsin-EDTA (0.25% trypsin, 1 mM EDTA), cell viability was checked by 0.4% trypan blue staining solution, counted via hemacytometer and re-seeded equal number of cells to maintain uniform cell growth rate and maintained for up to 8 weeks.
For cell viability assay, 10 μl of suspension culture was taken in eppendorf tube, added 190 μl of via count solution (viability counting solution) and incubated for 5 min at room temperature.
Focus on What Matters: Solutions Count!
has precisely solutions, counted multiplicity.
Figure 2 shows a typical case for c > 1 where dz ( m ) dm = 0 has an even number of real-valued solutions (counting multiplicities) which we denote them by m 1 − ≤ m 1 + < ⋯ < m q − ≤ m q + (in addition to m l,m u ).
Architecture and design questions tend to be naturally looser, and imaginative solutions count for a lot.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com