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Briefly, a total of 150 µL of the reaction mixture solution contained of 50 µL of 1% potassium ferricyanide, 50 µL of 0.2 M phosphate buffer (pH 6.6) and 50 µL of LJEO (100 500 µg/mL) or BHT/α-tocopherol (10 50 µg/mL) taken as reference standard compound (positive controls).
Briefly, a total of 100 µL of the reaction mixture solution contained of 10 µL of 1 mM ascorbic acid in 20 mM phosphate buffer, 10 µL of 1 mM FeCl3, 30 µL of PTVO (100 500 µg/mL) or BHT/α-tocopherol (10 50 µg/mL) taken as reference standard compound (positive controls) and 50 µL of bovine brain phospholipids (5 mg/mL).
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Patch pipettes of 5 10 MΩ resistance were filled with a solution containing of the following (in mM): 105 K-gluconate, 30 KCl, 10 HEPES, 10 phosphocreatine, 4 ATP, and 0.3 GTP, adjusted to pH 7.35 with KOH.
Cells were pretreated with solution containing of 2 mM 3-MA, 10 × 10 cells/ml PA-MSHA, or 3-MA in combination with PA-MSHA for 48 hours.
Stock solutions of 100 mM of the aldehyde of 2 in DMSO were prepared and added to diferric cADO to give final concentrations of 2 mM or 4 mM of the aldehyde of 2. Diferric cADO solutions containing of the aldehyde of 2 were incubated for 16 h at 4 °C immediately prior to crystallization.
Each single salt solution contained one of the four chemicals of NaCl, Na2SO4, MgCl2, MgSO4.
The standard phenol solution contained 1g of phenol in 95, 100, 105, 110 and 115ml of solutions made with sterile distilled water.
One-hundred milliliters of vitamin solution contained 10 mg of biotin and 20 mg of pyridoxol-HCl.
Transfection solution contained 2 μg of total DNA per mL of media.
Ringer's solution contained a racemic mixture of L- and D-lactate.
One week later, the phantom was filled with three aliquots of a solution containing 5% of iobitridol and weighed after each aliquot.
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