Mice were anesthetized with an intraperitoneal solution constituted by one part of ketamine 10% (Ketavet, Intervent Production s.r.l., Italy), one part of xylazine 20 mg/mL (Rompun, Bayer AG, Germany) and eight parts of sterile water, for a total of 0.2 mL.
The method was based on the formation of water-in-oil emulsion by mixing 2.0 mL of extraction solution constituted by 12% (w/v) Triton X-100 and 5% (v/v) HNO3 with 10 mL of sample.
The method was based on the formation of a water-in-oil emulsion by the addition of extractor solution constituted by 3.54% (m/v) Triton X-114 and 1.16 mol L− 1 HNO3 in the oil sample and further breaking of this emulsion by heating.
After an incubation time of 4 hours at 37°C, the formazan crystals formed by the metabolically active cells were solubilized by addition of 250 μl solution constituted by isopropanol, Triton X-100 and HCl.
In triplicates, methanolic extract (150 μl) of different concentrations at 10, 100 or 1000 μg/ml was added to 2850 μl of FRAP solution that constituted of 300 mM acetate buffer, 50 ml; 10 mM 2,4,6-tripyridyl-s-triazine (TPTZ), 5 ml; and 20 mM ferric chloride, 5 ml.
The oxidation reduction processes between these different oxides and the associated exchange of species with the solution constitute the main characteristic of this electrode.
This MSE-optimal analytical solution constitutes a special case of robust feedforward control filters for dynamic (frequency-selective) systems, previously developed in [26 28].
The proposed solution constitutes an integral part of an existing service provisioning platform targeted to the location based services (LBS) domain; however application to other service-oriented architectures is also possible.
Polar phases of films made from these latices were selectively impregnated with lithium salt solution, constituting dual-phase structures.
On the other hand, a similar study is done for a membrane immersed in solutions constituted by a mixture of NaCl and MgCl2.
