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The method is based on the formation of a cobalt-1-nitroso-2-naphthol complex in the micellar triblock copolymer compound solution constituted by PEO and PPO.
The method was based on the formation of water-in-oil emulsion by mixing 2.0 mL of extraction solution constituted by 12% (w/v) Triton X-100 and 5% (v/v) HNO3 with 10 mL of sample.
The method was based on the formation of a water-in-oil emulsion by the addition of extractor solution constituted by 3.54% (m/v) Triton X-114 and 1.16 mol L− 1 HNO3 in the oil sample and further breaking of this emulsion by heating.
After an incubation time of 4 hours at 37°C, the formazan crystals formed by the metabolically active cells were solubilized by addition of 250 μl solution constituted by isopropanol, Triton X-100 and HCl.
Mice were anesthetized with an intraperitoneal solution constituted by one part of ketamine 10% (Ketavet, Intervent Production s.r.l., Italy), one part of xylazine 20 mg/mL (Rompun, Bayer AG, Germany) and eight parts of sterile water, for a total of 0.2 mL.
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On the other hand, a similar study is done for a membrane immersed in solutions constituted by a mixture of NaCl and MgCl2.
Briefly, 2000 cells/well – plated in 96-well dishes – were treated separately with the respective siRNA solutions constituted by each siRNA pool as well as a fluorescent non-targeting siRNA, employed as negative control, diluted in Accell delivery media to a 1 μM final concentration.
Up to 10% of albumin solutions may be constituted by high-molecular-weight substances.
This solution constitutes the starting solution and the according columns are added to the pool Ω ′.
In the other solutions they are mainly constituted by boric acid (or borates, depending on the solution pH).
Impedance spectra were recorded before and after single cycle anodic polarization in dilute Harrison solution (DHS), which is constituted by a mixture of ammonium sulfate and sodium chloride.
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