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Subsequently, a detector antibody (rabbit anti- B. globigii), recognizing another epitope on the B.g. spore surface, was diluted in BSA diluent/blocking solution concentrate (1 15 dilution in distilled water) to a final concentration of 5 μg mL−1 and incubated at 37 °C with the captured spores before being washed several times (PBS + 0.5% Tween 20).
500 µl of UTM was then drawn from each tube and mixed with 500 µl of Lysis/Binding Solution Concentrate (LBSC; Ambion) in fresh tubes.
The trial compared a pre-mixed 'ready-to-use' solution of magnesium sulphate, with a reference drug, a commercially available infusion solution concentrate requiring dilution [ 36].
This ready-for-use solution (concentrate + water + hydrocarbonate = dialysate) serves as an electrolyte solution exposed to the 'water side' of the extracorporeal circuit [ 1, 2].
First, the contents of each vial of buffered wash solution concentrate were diluted with distilled water to a final volume of 1000 mL prior to use.
After immobilization of the capture antibodies, the remaining binding sites were blocked for 1 h at room temperature using a bovine serum albumin (BSA) diluent/blocking solution concentrate, diluted 1 10 in distilled water (Kirkegard and Perry Laboratories (KPL), Gaithersburg, MD).
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In consequence, the interior solution concentrates and needs an even lower temperature to freeze.
To this solution, concentrated H2SO4 (0.098 g, 1 mmol) was added and stirred well at room temperature (Scheme 1).
The results show that the total mass of the solution concentrates on a small set in the space of configuration.
Here MK2206, or normal saline were dissolved in 0.5% sodium carboxyl methyl cellulose solution, concentrated at 20 μg/ml.
NB cells were precipitated, put into the pre-warmed hypotonic solution, concentrated, fixed and then dropped onto clean microscope slides.
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