The primary rat hepatocytes were mixed with a prepolymer solution comprised of thiolated heparin and acrylated poly ethylene glycol) (PEG).
Briefly, HMO was synthesized by drop wise addition of 0.15 M Mn(NO3 2·4H2O to a solution comprised of 0.1 M KMnO4 and 0.2 M NaOH.
In accordance with the factorial matrix, gelispheres were formulated by titrating a combination polymeric solution comprised of sodium alginate, pectin and/or cellulose acetophthalate into an inducer solution (crosslinking agent) consisting of calcium and/or acetate ions.
A methanolic solution comprised of 95 mL of methyl alcohol and 5 mL of sulfuric acid was added at a molar ratio of 1 16 to the cashew nut shell oil.
Again, the samples were immersed in a solution comprised of H2O2 (30 %), HCl (37 %), and DI water in the volume ratio of 1 1 5 at 80 °C for 10 min to remove any metallic contamination.
The main hardening mechanism is a result of the precipitation of nanograined diamond membranes, which can be extracted by immersing the flame-hardened Cr C deposit in an etching solution comprised of 33 vol.% HNO3 and 67 vol.% HCl.
Most commercialized methods for TNT assay-with the exception of Cold Regions Research and Engineering Laboratory of the U.S. Army CRRELL) method-use proprietary chemicals, and the color stability and intensity are highly dependent on the composition of the organic solution comprised of acetone or methanol.
Before use, the two NTA groups of Cy3NTA were charged with 20 nmoles of NiCl2 in water to form a stock solution comprised of 500 µM Cy3NTA and 1 mM NiCl2 used for Cy3NTA titrations in functional assays.
The kit includes 2 syringes, one containing a solution comprised of factor XIII, plasminogen, plasma fibronectin and fibrinogen and a second syringe that contains a human thrombin solution.
As shown in Fig. 1, a stacking solution comprised of 1 mM carboxymethyl-β-cyclodextrin and 5 mM CAPS produced peak areas that were four to six times larger than those obtained with a stacking solution comprised of 1 mM carboxymethyl-β-cyclodextrin and 50 mM CAPS (see Electronic Supplementary Material (ESM) Table S1).
