A myriad of different solution cocktails and protective strategies have been offered and compared.
Cocktail, cocktail, cocktail, cocktail......
After washing in PBS, the sections were incubated with Envision solution (a cocktail of peroxidase-labeled polymer conjugated to goat anti-rabbit and goat anti-mouse immunoglobulins in Tris HCl buffer; DAKO Corp., Glostrup, Denmark) for 45 min at room temperature.
The resin was suspended in the cocktail solution and the solution was shaken for 1 h.
Individual tissue samples were weighed and then homogenized in five volumes of ice-cold RIPA buffer containing 25 mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS (Thermo Scientific, Waltham, MA, USA), protease inhibitor cocktail solution and phosphatase inhibitor cocktail solution (GenDEPOT, Barker, TX, USA).
For instance, search algorithms, which iteratively determine the optimal solutions (drug cocktails) in the parametric spaces (different drug combinations), can be applied for determining synergistic drug combinations.
pH enzyme membranes were prepared in almost the same way, only before solvent evaporation from the added membrane cocktail solution urease (15 mg/mL).
Cells were washed with cold phosphate buffer saline (PBS) and lysed with the radio-immunoprecipitation assay (RIPA) buffer containing 1 % proteinase inhibitor cocktail solution and 1 % phosphatase inhibitor cocktail solution (Sigma, St . Louis MO, USA).
Cells were harvested and lysed in RIPA buffer (GenDEPOT) with protease inhibitors (Xpert protease inhibitor cocktail solution, GenDEPOT) and phosphatase inhibitors (Xpert phosphatase inhibitor cocktail solution, GenDEPOT).
