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The slices were loaded with 5 µM Rhod 2-AM (Molecular Probe, Eugene, OR) in aCSF solution bubbled with O2 95% and CO2 5% gas mixture for 1 h in the dark at RT37.
A new solution, bubble-PCWS, which can make PCWS obtain both a high mass concentration and a good stability, has been presented (Gao et al. 2014).
When I asked why, the answer was as long as the solution bubbled, pus was present.
After angsting over the situation for days, a solution bubbled up: Let them bring their babies to work.
After 15 minutes in nutritive solution at room temperature, the ileum was sectioned (4.5 cm) and fixed in the perfusion chamber with 5 ml of aerated Tyrode solution bubbled with a gas mixture of 95% O2/5% CO2 at 37±0.5°C.
Ventral white matter strips were incubated for 1 h in Ca2+ free Krebs' solution (bubbled with 95% O2 and 5% CO2) containing 2 mM MgCl2 and 40 µM Oregon Green BAPTA2-AM (OG, Invitrogen), a cell permeant calcium indicator.
Isolated ventral white matter strips with a length of about 4 cm were maintained in Krebs' solution (bubbled with 95% O2 and 5% CO2) for 1 h prior to experiments.
Brain resections were collected in an ice-cold artificial cerebrospinal fluid solution bubbled with 5%CO2and95%5% O2.
Gastric tonometers, however, were usually validated in vitro in a solution bubbled with CO2.
Rat diaphragms were excised rapidly and kept at room temperature in Krebs–Henseleit solution bubbled with oxygen.
It is also possible that small items floating in the solution (bubbles, debris) could occasionally activate AF7.
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