Sentence examples for solution blocking from inspiring English sources

Internalization studies: The culture medium was removed, and the cells were washed once with 500 μL DMEM-F12 (5% BSA) before being left to equilibrate in 200 μL DMEM-F12 (5% BSA) at 37°C for a minimum of 15 min. Then, 25 μL (per well) of either DMEM-F12 (5% BSA) or of a 100-μM PMPA solution (blocking) was added, followed by the addition of 25 μL of 68Ga/177Lu-labelled PSMA ligand.

After transfer, blots were incubated in a solution (blocking solution) containing Tris-buffered saline (TBS), 0.5% (w/v) Tween-20, 1% (w/v) non-fat milk and 1% (w/v) bovine serum albumin.

Membranes were incubated with a 5% skim milk solution (blocking solution) for 1 h, and subsequently incubated with the indicated primary antibodies at 4 °C for 16 h.

After aspirating the coating solution, blocking solution was added to the wells for 2 hours and then the coated plates were washed three times.

Then embryos were permeabilized in 1% Triton X-100 in PBS for 4 h at 4°C, blocked with 1% BSA in washing solution (blocking solution), and incubated with the following antibodies: Oct4 (Santacruz Sc-8628), Cdx2 (Biogenex MU392A-UC), Nanog (Abnova PAB6837), Sox2 (Santacruz Sc-17320) and Gata6 (Abcam Ab22600) in blocking solution for 1 h at room temperature.

The membrane was soaked in protein blocking solution (Blocking One solution, Nacalai) for 30 min at RT before an overnight incubation at 4°C with primary antibody, also diluted in blocking solution.

Cells were cultured on cover slips, fixed with 4% paraformaldehyde (PFA) solution, blocked with 1 m M glycine (pH 7.5) and permeabilised for 4 min in 0.1% Triton.

Sections were first incubated with avidin and biotin solutions, blocking non-specific antibody binding.

200 μl TdT enzyme/reaction buffer, anti-digoxigenin antibody/blocking solution and blocking solution were added per cell-based microarray and 50 μl per 6-well plate coverslip.

So the team at Yik Yak came up with a technological, and rather creative, solution: block younger students from using the application by way of geo-fencing.

This chain is comprised of both the solution blocks (projects and platforms) as well as the "glue" (the standards and internetworking APIs that link them all together).