Exact(15)
The platform fits to the cup and operates in a small volume of the solution bathing the living epithelial cell layer (membrane) deposited on a porous support of the cup, which allows for effective monitoring of ion concentration changes.
To hunt for the chemical messenger, postdocs Karen Christopherson and Erik Ullian in Barres's lab grew astrocytes in culture and filtered the solution bathing the cells before adding it to neurons.
A heated stage maintained the temperature of the solution bathing the cells at ∼28 °C.
TG (1 μM) was perfused into the solution bathing the cells.
Bacterial cells were also counted in the Hoaglands solution bathing the roots and expressed as CFU/mL.
Variations in INa were measured with the loose patch-clamp method before and following alterations in the solution bathing the external face of the membrane.
Similar(44)
Temperature-controlled solutions are delivered directly to a single cell(s) through a multibarreled manifold that switches solutions bathing a cell in less than 1 s.
Excised patches were moved into flowing test solutions bathing the cytosolic membrane face.
The composition of solutions bathing the cytoplasmic face of the patch membrane contained (in mM) 140 K-methanesulfonate, 20 KOH, 10 HEPES, and 5 EGTA with the pH adjusted to values from 6.0 to 8.5 with HCl or KOH.
After 24 h, the root bathing solution was replaced to remove any remaining C-labelled 3-O-M-glucose.
After the plants were removed, the C content of the resulting root bathing solution was quantified using a Wallac 1404 scintillation counter (Wallac EG&G, Milton Keynes, UK).
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