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Exact(5)
Both were diluted in physiological salt solution as required.
Make up to 1 ml using nuclease free water [or 20 ng/μl yeast tRNA solution as required] (9.03 × 10 miRNA copies/μl).
Treatment included hydration with intravenous saline solution as required, an insulin regimen with insulin aspart administered subcutaneously every 2 hours, and oral carbohydrate intake if glucose levels were less than 250 mg/dL with persistent ketosis.
PE, ACh, SNP, LNNA and ADMA were from Sigma (St . Louis MO, U.S.A).; 10 mmol/L stock solutions were prepared in water and further diluted in physiological salt solution as required, except for ADMA, which was dissolved in water as 30 mmol/L stock solution.
The tops of the pots were wrapped in aluminum foil to minimize radiant heat absorption from the lights and the plants were uniformly fertilized with "Osmocote" (Chisso-Asahi Fertilizer Co., Tokyo, Japan) sustained-release fertilizer and a nutrient-complete ("Tune-up" TM, Plant Products Ltd., Brampton, ON, Canada) water-soluble solution as required.
Similar(55)
Thus there are three solutions, as required.
The volume was adjusted to 1,000 mL with distilled water to provide assay solutions as required.
Rats were anesthetized with an intraperitoneal injection of a ketamine hydrochloride (80 mg/kg) and xylazine (10 mg/kg) solution supplemented as required by intrajugular infusion of a 20% mixture of the ketamine/xylazine solution in dextran lactated Ringer's solution.
The advantage of this correlation is that it is explicit in z and thus does not require an iterative solution as is required by other methods.
Cold crystalloid potassium cardioplegic solution was used as required to maintain asystole.
All cell lines were maintained at 37°C/5% CO2 with regular subculturing using a trypsin/EDTA solution (SAFC Biosciences) as required.
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