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Phospho-STAT3 (Tyr 705) antibody (#9131, Cell Signaling, Danvers, MA) was diluted 1 1000 into blocking solution and sections were stained for 48 hr at 4°C.
Reaction was stopped using sodium thiosulfate solution and sections were counterstained with toluidine blue.
The colour was developed by 15 min of incubation with diamone benzidine solution, and sections were weakly counterstained with haematoxylin.
The colour was developed by 15 min of incubation with new fuchsin solution and sections were weakly counterstained with haematoxylin.
The colour was developed by 15 min incubation with DAB solution, and sections were counterstained weakly with haematoxylin.
The color was developed by 15 min incubation with DAB solution and sections were weakly counterstained with hematoxylin.
Similar(47)
At this time one tooth was randomly selected, removed from solution and sectioned through the lesion at three sites.
Section 3 presents our proposed solution and section 4 evaluates this algorithm.
Section 'Evaluation' contains an evaluation in terms of accuracy, performance and robustness of the solution and Section 'Related work' discusses related work.
Muscles were embedded in OTC freezing solution and sectioned sequentially from the distal tendinous insertion towards the mid belly region.
Brains were removed, bathed in chilled Hank's solution, and sectioned in the coronal plane with a motorized vibratome.
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